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Methylthioninium Chloride or Methylene Blue
CAS Number: 61-73-4 for Anhydrous and 7220-79-3 for Trihydrate
Molecular Formula: C16H18ClN3S-3H2O Trihydrate
Molecular Weight: 373.9 Trihydrate
Methylthioninium Chloride or Methylene Blue
Methylene Blue USP Grade Specifications
Methylene Blue
C16H18ClN3S-3H2O -- 373.90
Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride, trihydrate.
C.I. Basic Blue 9 trihydrate CAS 7220-79-3
Anhydrous 319.86 CAS 61-73-4
Methylene Blue contains not less than 98.0 percent and not more than 103.0 percent of C16H18ClN3S, calculated on the dried basis.
Identification: Infrared Absorption 197K.
Loss on drying: Dry it at 75 and at a pressure not exceeding 5 mm of mercury for 4 hours: it loses between 8.0% and 18.0% of its weight.
Residue on ignition: not more than 1.2%.
Arsenic: Prepare the Test Preparation by mixing 0.375 g with 10 mL of water in the arsine generator flask. Add 15 mL of nitric acid and 5 mL of perchloric acid, mix, and heat cautiously to the production of strong fumes of perchloric acid. Cool, wash down the sides of the flask with water, and again heat to strong fumes. Again cool, wash down the sides of the flask, and heat to fumes. Cool, dilute with water to 52 mL, and add 3 mL of hydrochloric acid: the resulting solution meets the requirements of the test, the addition of 20 mL of 7 N sulfuric acid specified for Procedure being omitted. The limit is 8 ppm.
Copper or zinc: Ignite 1.0 g in a porcelain crucible, using as low a temperature as practicable, until all of the carbon is oxidized. Cool the residue, add 15 mL of 2 N nitric acid, and boil for 5 minutes. Filter the cooled solution, and wash any residue with 10 mL of water. To the combined filtrate and washing add an excess of 6 N ammonium hydroxide, and filter the solution into a 50 mL volumetric flask. Wash the precipitate with small portions of water, adding the washings to the filtrate, dilute the solution with water to volume, and mix. To 25 mL of the solution add 10 mL of hydrogen sulfide TS: no turbidity is produced within 5 minutes (absence of zinc). Any dark color produced does not exceed that of a control prepared by boiling a quantity of cupric sulfate, equivalent to 200 @g of copper, with 15 mL of 2 N nitric acid for 5 minutes and by treating this solution as directed above, beginning with “Filter the cooled solution” (0.02% of copper).
Chromatographic purity: To pass the test.
We also supply Methylthioninium Chloride or Methylene Blue BP Ph Eur Grade.
Please visit SDS Safety Data Sheet of Methylthioninium Chloride or Methylene Blue Suppliers.
American Molecules, also known as ammol.org is a distributor, supplier and manufacturers' representative of all types of Pharmaceuticals, Functional Ingredients, Excipients and Specialty Chemicals in Texas USA. Our principals manufacture supply and export USP NF BP, Ph Eur, etc grades of chemicals pure and reagent grade, mineral fortifiers, FCC food grade. Tailor made particle size and customized specifications are offered. The principal's facility is having one or more of the certifications like FDA approval and GLP, cGMP, ISO9001, ISO14001, ISO/IEC 17025, ISO22000, FSSC 22000, ISO45001, FSSAI, Kosher, HALAL, COPP, WHO-GMP certified and Written Confirmation (WC) for export to Europe is available. The manufacturers suppliers and exporters observe WHO Good Manufacturing Practices and Good Laboratory Practices.
Suppliers and Manufacturers' Representative:
9910 Bent Oak Dr
Houston, TX 77040, USA
Call Toll Free: 1-855-55-AMMOL 1-855-552-6665
Email: info@ammol.org