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Vitamin A or Retinol USP BP Ph Eur FCC Food Grade Supplier Exporter Manufacturers' Representative |
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Vitamin A or Retinol
CAS Number: 68-26-8
Molecular Formula: C20H30O
Molecular Weight: 286.46
Vitamin A or Retinol
Vitamin A USP Grade Specifications:
DEFINITION
Vitamin A contains a suitable form of retinol (C20H30O; vitamin A alcohol) and possesses vitamin A activity equivalent to NLT 95.0% of that declared on the label. It may consist of retinol or esters of retinol formed from edible fatty acids, principally acetic and palmitic acids. It may be diluted with edible oils; or it may be incorporated in solid, edible carriers, or excipients; and it may contain suitable antimicrobial agents, dispersants, and antioxidants.
IDENTIFICATION
A. Color Reaction with Antimony III
Sample solution: Equivalent to 6 µg/mL of retinol in chloroform.
Analysis: To 1 mL of the Sample solution add 10 mL of antimony trichloride.
Acceptance criteria: A transient blue color appears at once.
B. Thin-Layer Chromatography: To pass the test.
Absorbance Ratio: To meet the requirement.
Vitamin A FCC Food Grade Specifications:
All-trans-Retinol
DESCRIPTION
Vitamin A occurs as a light yellow to red oil that may solidify on refrigeration (liquid form). In solid form it may have the appearance of the diluent that has been added to it. It is a suitable form or derivative of retinol (C20H30O; Vitamin A alcohol). It usually consists of retinol or esters of retinol formed from edible fatty acids, principally acetic and palmitic acids, or mixtures of these. It may be diluted with edible oils, or it may be incorporated in solid edible carriers, extenders, or excipients. It may contain suitable preservatives, dispersants, and antioxidants, providing it is not to be used in foods in which such substances are prohibited. In liquid form it is very soluble in chloroform and in ether, it is soluble in absolute alcohol and in vegetable oils, but it is insoluble in glycerin and in water. In solid form it may be dispersible in water. It is unstable in air and light.
Function: Nutrient.
Identification:
A. Dissolve an amount of sample equivalent to about 6 µg of retinol in 1 mL of chloroform, and add 10 mL of antimony trichloride. A transient blue color appears at once.
B. Assemble an apparatus for Thin-Layer Chromatography, using chromatographic silica gel as the adsorbent and a 4:1 mixture of cyclohexane:ether as the solvent system. Prepare a Standard Solution by dissolving the contents of 1 capsule of USP Vitamin A Reference Standard in sufficient chloroform to make 25.0 mL.
If the sample is in liquid form, dissolve a volume representing approximately 15,000 International Units in sufficient chloroform to make 10 mL of Sample Solution. If the sample is in solid form, weigh a quantity representing approximately 15,000 International Units, place in a separator, add 75 mL of water, heat, if necessary, to dissolve the carrier, and cool. Shake vigorously for 1 min, extract with 10 mL of chloroform by shaking for 1 min, and centrifuge to clarify the chloroform extract.
At the starting point of the chromatogram, apply 0.015 mL of the Standard Solution and 0.01 mL of the Sample Solution. Develop the chromatogram in the chromatographic chamber lined with filter paper dipping into the solvent mixture. When the solvent has ascended 10 cm, remove the plate, allow it to dry in air, and spray it with antimony trichloride. The blue spot formed is indicative of the presence of retinol. The approximate Rf values of the predominant spots, corresponding to the different forms of retinol, are 0.1 for the alcohol, 0.45 for the acetate, and 0.7 for the palmitate.
Assay: Not less than 95.0% and not more than 100.5% of the Vitamin A activity declared on the label.
Absorbance Ratio: (corrected/observed at 325 nm) Not less than 0.85.
Lead: Not more than 2 mg/kg.
We also supply Vitamin A BP Ph Eur Grade as per the latest monograph.
Please visit SDS Safety Data Sheet of Vitamin A or Retinol Suppliers.
American Molecules, also known as ammol.org is a distributor, supplier and manufacturers' representative of all types of Pharmaceuticals, Functional Ingredients, Excipients and Specialty Chemicals in Texas USA. Our principals manufacture supply and export USP NF BP, Ph Eur, etc grades of chemicals pure and reagent grade, mineral fortifiers, FCC food grade. Tailor made particle size and customized specifications are offered. The principal's facility is having one or more of the certifications like FDA approval and GLP, cGMP, ISO9001, ISO14001, ISO/IEC 17025, ISO22000, FSSC 22000, ISO45001, FSSAI, Kosher, HALAL, COPP, WHO-GMP certified and Written Confirmation (WC) for export to Europe is available. The manufacturers suppliers and exporters observe WHO Good Manufacturing Practices and Good Laboratory Practices.
Suppliers and Manufacturers' Representative:
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Houston, TX 77040, USA
Call Toll Free: 1-855-55-AMMOL 1-855-552-6665
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